mRNA Sequence

1. Write the mRNA sequence, transcribing from the template strand only (it is in italics). Note that the RNA polymerase does not “know” that a base is “mutated”, when compared to the original base, so it would transcribe according to the usual base-pair rules. Therefore, even though you know there is mutation in the DNA, write out your mRNA sequence using the entire mutated template strand according to the base-pair rules for DNA bases binding with RNA bases.2. Use the genetic code to write out the corresponding a-a’s of the mutant protein based on the mRNA transcript that you just wrote. Students find it helpful to separate the mRNA sequence ino its triplet codons by drawing a line between every 3rd RNA nucleotide. Remember, “do what a ribosome would do” when translating your mRNA sequence into a-a’s. So, if you encounter a stop codon (e.g. one that was CREATED by the mutation), do not translate beyond that point, EVEN though a stretch of mRNA still remains. Points will be deducted if I see extra a-a’s.3. Conclude with a statement indicating whether the mutant protein, as compared to the original protein on pg. 29, is “functional” or “nonfunctional” (either one of these words is required for full marks). Add a phrase/sentence explaining your logic behind choosing functional or nonfunctional. Thus, consider how many a-a’s were actually changed because of the mutation.4. Upload your completed assignment by submitting a pdf file (separate pdf files, one for each page, are acceptable too). Write your responses using actual copies of pg. 29 to 31 (you have the pdf of the Learning objectives so can print out clean copies of those pages if you need to). Grading goes a lot quicker if I have those pages to review…the quicker I can grade, the faster I can return them to you.